properties of matter : Containing 4.8x10-4 mol/L BCIP and 5.1x10-4 mol/L NBT. No dilution before use is necessary. BCIP/NBT Solution is a single strength, ready-to-use substrate for the detection of Alkaline Phosphatase. Its dark blue, insoluble end-product is ideal for use in immunohistochemistry, Northern/Southern hybridizations that use Alkaline Phosphatase labeled probes.