properties of matter : Freeze-dried powder. Protease S specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. with hemoglobin as the substrate. Autolysis occurs at temperature greater than 40°C. The enzyme is fully active in USP 0.2% SDS. Stable for 12 months at 2~8°C. One Unit causes a change of 0.001 A280 nm per minute at 37°C, pH 7.8 using casein as the substrate. Chromatographically purified accourding to Drapeau, G., Boily, Y., and Houmard, J. JBC, 247, 6720(1972).

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