- The EcoBand Protein Marker(5-250kDa) is a combination of 13 pre-stained proteins with 5-color molecular weights from 5 to 250 kDa. The 13 recombinant proteins are covalently coupled with blue chromophore, while a red band at 80 kDa, a green band at 25 kDa, a newly designed yellow band at 15 kDa and a purple band at 250 kDa serve as reference bands. The EcoBand Protein Marker(5-250kDa) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
- Name : EcoBand Protein Marker(5-250kDa)
- Cat No : Q161302-0500
- Guide for Molecular Weight Estimation (kDa) :Refer to the EcoBand Protein Marker(5-250kDa) patterns in various electrophoresis conditions: 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
- Note : The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
- Storage Buffer : 0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.
- Quality Control : 5 μl of EcoBand Protein Marker(5-250kDa) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
- Storage : Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. Stable for up to 24 months at -20°C.
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